Housekeeping gene expression during fetal brain development in the rat-validation by semi-quantitative RT-PCR.

Study of sex differences in the expression of β-actin gene in various tissues of the rat

Introduction: Beta-actin is a housekeeping gene, which is used as an internal standard in various biological studies. Recently, it was reported that the expression of commonly used housekeeping genes including ß–actin changes in disease states or under certain experimental conditions. Based on the reports about the effects of sex hormones on the expression of the ß- actin gene, in this study...

Semiquantitative RT-PCR analysis to assess the expression levels of iNOS in chicken macrophages

Abstract: iNOS is inducible by a variety of factors related to inflammation and referred to as inducible NOS(iNOS). It is regulated at the level of gene expression; once expressed, it produces NO at a high rate. iNOS gene-expression profiling is an important tool in understanding molecular markers of the responses of cells and tissues to external factors. In this article a semiquantitative reve...

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)

Expression pattern analysis of TomPRO2 and LaPA1 genes in tomato under in vitro salt stress by Semi-quantitative RT-PCR

The expression pattern of TomPRO2 and LaPA1 genes in two tomato (Lycopersicon esculentum) cultivars named as Isfahani and Shirazi under in vitro salt stress were investigated. Four to six weeks old in vitro grown seedlings were transferred on MS medium containing 0, 80 and 160 mM NaCl and untreated plants were used as control. RNA was extracted from root and leaf and then cDNA was synthesized. ...

Semi-quantitative reverse-transcriptase polymerase chain reaction: an approach for the measurement of target gene expression in human brain.

Polymerase chain reaction (PCR) is a very powerful tool for qualitative evaluation of nucleic acids due to its high efficiency and convenience. Together with the reverse transcription (RT) reaction, the PCR method has been widely applied to the quantitative measurement of DNA and RNA messages. Since RT-PCR is much more sensitive than all of the traditional methods for quantification of mRNA, in...